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Parasites

Parasites

REVIEW ARTICLE PUJ
Vol. 4, No. 1, 2011
ISSN: 0258-3216
Personal non-commercial use only. PUJ copyright © 2011. All rights reserved PUJ; 2011, 4(1): 3-14
Herbal Medicine and Parasitic Diseases
Samia E. Etewa1, Sherif M. Abaza2
Parasitology Department, Faculty of Medicine, Zagazig1 and Suez Canal2 Universities, Ismailia, Egypt
Received: August, 2010 Accepted: October, 2010
Examples of Known Herbs with Antiparasitic Effects
Allium cepa; Onion (Al-Basal)
Two sets of compounds make up the majority of onion's
known active constituents: Sulphur compounds such as
allicin and allyl propyl disulphide and avonoids such as
quercetin. Allicin exhibits its antimicrobial action mainly
by immediate and total inhibition of RNA, DNA and
protein synthesis
(10)
.
In a study conducted in 2004, all tested strains of
Leishmania (major, donovani and mexicana) were found to
be sensitive in vitro to the onion juice in the promastigote
stage
(11)
. Aqueous onion extract is similar to metronidazole
in inhibiting both multiplication and motility of T.
vaginalis
(12)
. A. cepa oil has an antihelminthic effect in rats
experimentally infected with T. spiralis and increases the
production of antibodies generated during life cycle of the
parasite
(13)
. Recently, effectiveness of A. Cepa extract in
eyelids inammation caused by Demodex folliculorum
was tested with promising results
(14)
. Onion reportedly
exerted different physiological changes in snails of
B. alexandrina leading to decrease in glucose and glycogen
which are the snail energy fuel and inhibited the phenol
oxidase enzyme which led to
disturbance in egg-shell
formation
(15)
. Some adverse effects have been reported
where higher intakes may worsen existing heartburn and
there are also isolated reports of allergy manifesting as
skin rash and red, itchy eyes
(16)
.
INTRODUCTION
 
Herbal medicine (also known as traditional, folk
and alternative medicine) comprises medical
knowledge systems that developed over generations
within various societies before the era of modern
medicine. WHO denes traditional medicine as the
health practices, approaches, knowledge and beliefs
incorporating plant, spiritual therapies and manual
techniques, applied singularly or in combination
to treat, diagnose and prevent illnesses or maintain
well-being(1). Ancient Egyptian medicine, 1000
Before Christ (BC), was known to use garlic,
opium, castor oil, coriander, mint and other herbs
for medicinal purposes and Indian medicine used
turmeric (Curcumin) possibly as early as 1900
BC(2). N. sativa seeds have been used as a traditional
medicine for the treatment of a variety of sicknesses
including parasitic diseases(3). The scope of herbal
medicine sometimes extended to include fungal and
bee products, as well as minerals, shells and certain
animal parts(4). It is estimated that 20,000 species of
higher plants are used medicinally throughout the
world. In some Asian and African countries, up to
80% of the population rely on traditional medicine
for their primary health care needs(1).
The emergence of parasites resistant to current
chemotherapies highlights the importance
of plant essential oils as novel anti-parasitic
agents; e.g., in schistosomiasis(5), malaria(6) and
visceral leishmaniasis(7). Some plant oils have
immunomodulatory effects that could modify host-
parasite immunobiology and the lipid solubility of
plant oils might offer alternative, transcutaneous
delivery routes(8). In addition, the safety and use
of plant essential oils in drug resistant cases are
considered the most advantages. However, lack of
proper understanding of plant and drug interactions
have led to adverse reactions that are sometimes life
threatening or lethal(9). Furthermore, adulteration
or counterfeit herbs can also be a health hazard, as
WHO conrmed that inappropriate use of traditional
medicines or practices can have negative or dangerous
effects(1).
The present review is an analysis to through light on
different herbs with their active compunds that could
be used as drug targets in parasitic diseases. It also
discusses some herbs with
repellent activity and
molluscicidal effects.
 
Corresponding author: Sherif M. Abaza, smabaza@hotmail.com
Keywords: Herbal Medicine, Parasitic Diseases, Treatment, Repellents, Molluscicides.
 
 
 
Herbal Medicine and Parasites
4
Allium sativum; Garlic (Al-Tom)
The active constituents of garlic exceed 200 chemicals.
It contains sulphur compounds (allicin, alliin and
agoene), volatile oils, enzymes (allinase, peroxidase
and miracynase), carbohydrates (sucrose and glucose),
minerals (selenium), amino acids such as cysteine,
glutamine, isoleucine and methionine, bioavonoids
such as quercetin and cyanidin, Allistatin I and Allistatin
II and vitamins A, B1, B2, C, E and niacin
(17)
. The main
antimicrobial effect of allicin is due to its chemical
reaction with thiol groups of various enzymes e.g.
alcohol dehydrogenase and DNA polymerase which
affect the essential metabolism of cysteine proteinase
activity involved in the virulence of parasites
(18)
.
Garlic appears to be safe during pregnancy and breast-
feeding. Some sensitive people may experience
heartburn and atulence
(19)
.
Antiprotozoal effects: Several studies were conducted
to investigate the efcacy of garlic in giardiasis. There
were reports of complete relief of clinical symptoms
within 36 hours
(20)
, loss of agellar movement and
motility with trophozoites swelling leading to decreased
ability to adhere to host cells
(21)
, inhibition of Giardia’s
cysteine proteases activity and excretory/secretory
products resulting in reduction of GIT symptoms
(22)
. The
therapeutic effect of allicin was evaluated against C.
cayetanensis and the results showed that Tomex (10 mg/
Kg/day of garlic extract) caused signicant reduction
in oocyst shedding and improvement of intestinal
pathological changes (100% in immunocompetent and
80% in immunosuppressed mice)
(23)
.
The circumsporozoite protein (CSP), which is the
major surface protein of Plasmodia sporozoites, is
proteolytically processed by a parasite-derived cysteine
protease and this event is temporally associated with
sporozoite invasion of host cells. At low concentrations,
allicin inhibited CSP processing and prevented
sporozoite invasion of host cells in vitro. In vivo,
mice injected with allicin had decreased Plasmodia
infections compared to
controls. Allicin was also
tested on erythrocytic
stages where a
4-day regimen
of allicin administered
either orally or intravenously
signicantly increased the survival of infected mice
by 10 days
(24)
.
In mice infected with L. mexicana, intraperitoneal
injection of garlic extract or its protein fraction
augmented parasite engulfment and destruction of
intracellular amastigotes by macrophages. Modulation of
the immune response through macrophages activations
was postulated as a mechanism of action of garlic
(25)
.
Another explanation claimed that garlic extract reduced
macrophage infection through induction of nitric oxide
(NO) production in vitro. Therefore, it may act on both
T cells and macrophages to stimulate IFN-γ production
and NO synthesis for parasite killing
(26)
.
In a study conducted in 2007, Eimeria stiedae induced
marked hepatic histopathological alterations in rabbits
that were not treated with garlic, versus those treated
with garlic. The effect of methanol garlic extract
in a concentration of 3.90 mg/ml, on the growth of
Acanthamoeba castellanii and its cytotoxicity on
corneal
cells was studied in vitro. These ndings
indicated its amoebicidal, as well as cysticidal effects
on trophozoites and cysts, with no cytotoxicity to
corneal cells
(28)
. In addition, aqueous garlic extract
proved similar to metronidazole in inhibiting both
multiplication and motility of T. vaginalis
(13)
.
Antihelminthic effects: Aqueous garlic extract, with
its potent free radical scavenging and antioxidant
properties, seemed to be a highly promising agent in
protecting hepatic tissue against oxidative damage due
to S. mansoni infection
(29)
. Garlic efcacy was highest in
the group treated with garlic before and after bilharzial
infection and resulted in various ultrastructural
alterations in the surviving adult worm’s tegument
(30)
.
Results of a study conducted in 2008 showed that
chloroform garlic extract exerted the highest effect
on the viability of hydatid cyst protoscoleces in vitro,
in comparison with garlic aqueous extract and hydro-
alcoholic garlic extract
(31)
.
Garlic as repellent and its acaricidal effect:
The repellent effect of garlic oil was evaluated against
female Phlebotomus bite. A dose dependent anti-feeding
effect, which was 100% effective at 1% concentration,
was obtained
(32)
. Topical application of 10% garlic juice
by spraying, effectively decreased Northern fowl mite
(NFM, Ornithonyssus sylviarum) infestation in laying
hens. Humans could be bitten by these mites
and suffer
irritation and allergic reactions
(33)
.
Garlic as molluscicide: The effect of the plant-derived
molluscicides Annona squamosa and Lawsonia inermis
combined with A. sativum on the
reproduction of the
snail Lymnaea was studied. A signicant reduction in
the fecundity, hatchability and survival of young snails
was observed
(34)
.
Artemisia (Al-Sheeh)
There are about 300 species in the Artemisia genus.
Two of them, sweet annie (Artemisia annua) and
wormwood (Artemisia absinthium), are of medical
importance in treatment of some parasitic diseases.
The active constituents in the rst one are artesunate,
dihydroartemisinin, artemether and arteether, while the
second contains aromatic oils (thujone and isothujone)
and strong bitter agents (absinthin and anabsinthin).
Adverse effects associated with artemether treatment
were abdominal pain, anorexia, nausea, vomiting,
diarrhoea and CNS involvement (headache and
dizziness)
(35)
. Longer-term use of wormwood (over 4
weeks) can cause nausea, vomiting, insomnia, restlessness,
vertigo, tremors and seizures
(36)
. In addition, it is not
recommended during pregnancy and breast-feeding
(37)
.
 
 
Etewa and Abaza
5
Artemisinin (ART) derivatives: Mass drug
administration schemes in malaria control should
ideally use more than one drug, preferably combinations
including a rapid-acting schizonticidal drug, such
as ART, as well as primaquine that can kill sexual
and liver stages to prevent transmission. This could
be considered as an alternative strategy for malaria
control, and in
combination with other anti-malarial
measures may provide a tool for malaria elimination and
eradication
(38)
. ART combination therapy is the rst-line
treatment for uncomplicated P. falciparum malaria in
most malaria-endemic countries
(39)
. All ART compounds
induce very rapid reduction of parasitaemia, starting
almost immediately after administration. The proposed
mechanism of action involves cleavage of endoperoxide
bridges by iron producing free radicals which damage
biological macromolecules causing oxidative stress in
parasitized cells
(40)
. Pure artemisinin has a low solubility
in water, oil and therefore it could be administered
orally, rectally and intramuscularly. Intramuscular
artesunate is easier to administer but is associated with
hypoglycaemia
(41)
. Unfortunately, oral administration is
often not possible in patients
with severe malaria, due
to extreme vomiting; hence the development of several
semi-synthetic ART derivatives
(42)
.
Artemisinin also showed anti-leishmanial activity for
both promastigotes and amastigotes and was accompanied
by a high safety index. The leishmanicidal activity of
artemisinin was mediated
via apoptosis as evidenced
by externalization of
phosphatidylserine, loss of
mitochondrial membrane potential and cell-cycle arrest.
These data indicated promising anti-leishmanial activity
mediated by programmed cell death
(43)
.
Moreover, several ART derivatives were shown to be
effective against T. gondii in vitro
(44)
as well as in vivo
in a murine model of reactivated toxoplasmosis
(45)
.
The mechanism of action is uncertain. However, it
was suggested that they may inhibit sarcoplasmic-
endoplasmic reticulum (ATPase), thus disrupting
calcium homeostasis by increasing the periodicity of
calcium oscillations and inducing recurrent, strong
calcium spikes
(44)
. In addition, these new ART derivatives
have the ability to inhibit multiple steps of T. gondii
lytic cycle; they effectively inhibited T. gondii growth,
tachyzoite replication, attachment to and invasion of
host cells
(46)
.
The ART derivatives were shown to possess a broad
spectrum of activities against several helminthic
infections. Combination between artemether and
praziquantel showed highest efcacies against juvenile
and adult worms of S. haematobium
(47)
. Scanning and
transmission microscopic observations indicated that
artemether induces extensive damage to juveniles and
adults of different Schistosoma spp.
(48)
. In addition,
signicant progress has been made with artemether use
for chemoprophylaxis in schistosomiasis, as immature
stages of S. mansoni are more prone to oxidative
killing than mature worms which probably participates
in the mechanism of anti-schistosomal action of
ART
(49)
. The efcacy and safety of artemether was
studied in sheep infected with Fasciola spp. A single
intramuscular dose of artemether signicantly reduced
both egg and worm burdens. There were no adverse
events, however, two abortions were observed 7 days
post treatment(50). Recently, a study was carried out to
determine the morphological changes to F. hepatica
after in vivo treatment with artemether and the results
showed increased disruption of the tegumental system,
with isolated patches of surface blebbing and reduced
production of secretory bodies by the tegumental cells
(51)
.
Wormwood: A. absinthium extracts were found to be
effective against the enteral (adult) and parenteral (larva)
phases of trichinellosis
(52)
. In addition, oil extracts were
tested in the laboratory against host-seeking nymphs of
Ixodes ricinus Linnaeus (Acari: Ixodidae). The results
showed 62-70% repellent ability and hence could be
used in control of arthropods of medical, veterinary or
agricultural importance
(53)
.
Commiphora molmol; Myrrh (Al-Mor)
All the three main constituents of myrrh; resin,
gum and
volatile oil are important in myrrh's
activity as herbal
medicine
(54)
. The mechanism of the anti-schistosomal
action of myrrh on S. mansoni is not fully understood.
However, it has been attributed to permanent muscle
paralysis of the worms leading to their shift to the
liver where destruction takes place
(55)
. Meanwhile,
results of studies published on the efcacy of myrrh
in the treatment of S. mansoni-infected mice are
greatly conicting. Several experimental and clinical
studies reported signicant parasite reductions and
marked ultrastructural changes after treatment of S.
mansoni-infected mice with Mirazid
®(56,57)
or under eld
conditions
(58)
. However, other studies negated myrrh
efcacy in the treatment of schistosomiasis in either
experimental
(59)
, or clinical studies
(60)
.
There is also such debate over the efcacy of Mirazid
®
 
for the treatment of fascioliasis. Complete cure rate
was achieved in experimentally infected rabbits with
oral dose of 20 mg/day for 6 consecutive days
(61)
.
On the contrary, results obtained for experimentally
infected sheep treated with Mirazid
®
appeared similar
to those for untreated infected animals
(62)
. On the other
hand, Mirazid
®
was reported to be effective against
D. dendriticum
(63)
, H. heterophyes
(64)
and H. nana
(65)
.
Regarding its antiprotozoal efcacy, Mirazid
®
was
effective against cryptosporidiosis
(66)
and trichomoniasis
in metronidazole-resistant infected females
(67)
.
Myrrh proved to have insecticidal activity against
mosquito larvae of Culex pipiens and Aedes caspius
(68)
.
In addition, the molluscicidal activity of myrrh was
evaluated in several studies on B. alexandrina, B.
truncatus and L. cailliaudi and the results revealed
signicant lethal and ovicidal effects
(69,70)
.
 
 
Herbal Medicine and Parasites
6
Curcuma longa; Turmeric (Al-Korkom or Curcumin)
As turmeric is a very cheap, easily available, effective
and acceptable mode of treatment
in developing
countries, with no toxic or adverse reaction, it was used
as paste for the treatment of scabies, with 97% cure rate
within 3-15 days of treatment
(71)
. The active
constituent known as curcumin showed a wide
range of therapeutic actions. When used in the
recommended amounts, turmeric is generally safe.
Some herbal books recommend not taking high
amounts of turmeric during pregnancy as it may cause
uterine contractions and patients with gallstones or
bile passages obstruction are advised to consult their
healthcare practitioner before using turmeric
(72)
.
Curcumin had a signicant effect on the progression
of experimental cerebral malaria. Survival of treated
mice was signicantly increased and development of
cerebral malaria was either delayed or prevented
(73)
.
Possessing antiprotozoal effects, turmeric as well
proved to (1) show cytotoxicity against T. brucei
(74)
,
(2) have leishmanicidal activity in vitro
(75)
, (3) inhibit
G. lamblia trophozoite growth and adhesion, by more
than 50%, in dose and time dependent manner
(76)
and (4) be an attenuating agent against T. gondii
tachyzoites in the peritoneal uid of mice
(77)
.
Studies were designed to evaluate the schistosomicidal
activity of curcumin in vivo as well as immuno-
modulation of granulomatous inammation in acute
schistosomiasis mansoni. It was found that praziquantel
was more effective in lowering worm burden while
curcumin was more potent in reducing egg count
(78)
.
The mechanism by which curcumin might function as
a schistosomicidal agent is unclear and needs further
investigation. In another study, curcumin was effective
in reducing both male and female worms and tissue-egg
burdens, hepatic granuloma volume and liver collagen
content. In addition, curcumin treatment modulates
cellular and humoral immune responses of infected
mice
(79)
. On the other hand, curcumin is effective
in the treatment of many inammatory diseases. Its
efcacy on reducing the histopathological changes
of opisthorchiasis in hamsters was evaluated. Results
showed reduced inammatory cells aggregation
surrounding the hepatic
bile ducts, leading to reduced
risk factors of cholangiocarcinoma development
(80)
.
For molluscicidal activity, turmeric was evaluated
against B. alexandrina and the results showed that its
30 ppm extract stopped snail egg laying and caused
deformity of all embryonic stages
(81)
.
Nigella sativa; Nigella (Black Seed or Habbat
Al-Barakah)
Nigella oil contains saponin, nigellidine and
nigellone
and its seeds contain thymoquinone, monoterpenes
such as p-cymene and α-pinene
(82)
. Several mechanisms
were described for the activity of black seeds such as
its protection against hepatotoxicity
(83)
, its antioxidant
role
(84)
and stimulation of immune system
(85)
. The results
of the studies conducted in 2007 proved that its aqueous
extract could be useful in the treatment of B. hominis
(86)
 
and as an anti-malarial drug
(3)
. In the following year, its
inhibitory effect against T. vaginalis was investigated
in vitro and the results showed remarkable inhibition of
trophozoites growth and motility
(87)
.
The antioxidant and anti-schistosomal activities of N.
sativa oil was studied in control and S. mansoni-infected
mice. Remarkable reduction in worms, tissue eggs and
alteration in oogram pattern were recorded in all the
treated groups. The data pointed to the importance
of these seeds as a promising agent to complement
schistosomiasis specic treatment
(29)
. Its antiparasitic
effect was studied also in H. nana-infected mice, where
it was found to reduce the infection starting from 2
nd
 
day of the treatment
(85)
. Moreover, its prophylactic
treatment prior to T. spiralis infection was effective
against both adult worms and muscle larval count in
infected rats
(14)
.
Zingiber ofcinale; Ginger (Ganzabil)
The main constituents of ginger are the gingerols,
shogaols, zingerone and paradol
(88)
. The anti-amoebic
effect of a crude drug formulation against E. histolytica
was studied in comparison to metronidazole. Results
showed that the product had a minimal inhibitory
concentration of 1000 mg/ml as compared with 10 mg/
ml for metronidazole
(89)
. In vitro, a concentration of
200 mg of ginger extract killed almost all Schistosoma
worms within 24 hrs
(90)
. The anti-helminthic activity
of ginger was reported also in sheep naturally
infected with mixed species of GIT nematodes e.g. T.
colubriformis, Strongyloides papillosus and Trichuris
ovis
(91)
. Another study conducted in 2006 showed
that there was signicant reduction in intestinal adult
worms
and muscle larval count in mice infected with
T.
Spiralis
(92)
. In addition, there was microlaricidal
activity of aqueous extracts of ginger when administered
to dogs infected with D. immitis larids(93).
Ginger essential oils proved to have repellent results
against host-seeking chiggers (scrub typhus) of
Leptotrombidium imphalum(94) and ovicidal and repellent
effects against different mosquitoes as Anopheles
stephensi, Aedes aegypti and Culex quinquefasciatus(95).
In addition, its petroleum ether extract has larvicidal
activity against A. aegypti and C. Quinquefasciatus(96).
As a molluscicidal agent, gingerol and shogaol exhibited
potent effects on Biomphalaria glabrata, indicating
their ability to interrupt Schistosoma transmission(97).
Results of in vivo exposure of Lymnaea acuminata to
gingerol
proved its effect on snail neurotransmission
mechanisms, either separately or through a complex
interaction
between the different neurotransmitters
(98)
.
 
 
Etewa and Abaza
7
Berberis vulgaris, Berberis Aristata; Barberry
The active constituent in barberry is berberine which
is an alkaloid having antibacterial, anti-amoebic, anti-
fungal, anti-helminthic and leishmanicidal properties. Its
reported main side effect was interference with normal
liver function in infants. Strong standardized extracts
may cause stomach upset and should be used for no
more than two weeks continuously. Other symptoms of
excessive berberine intake include lethargy, nose bleed,
skin and eye irritation and kidney irritation
(71)
.
Berberine is a useful drug for the treatment of visceral as
well as cutaneous leishmaniasis. In L. donovani infection,
it inhibited in vitro multiplication of amastigotes
in macrophage culture and their transformation to
promastigotes in cell free culture
(99)
, while 1% berberine
sulphate inoculated intra-lesionally was found to be
highly effective against cutaneous leishmaniasis in
domestic dogs
(100)
. In addition, crude extract formulation
including berberine had a maximum cure rate of 73%
at a dose of 800 mg/kg/day in hepatic amoebiasis
(101)
.
The results of a study conducted in patients with
chloroquine-resistant malaria indicated that berberine
was more effective in clearing the parasite than both
tetracycline and cotrimoxazole and that the combination
of pyrimethamine and berberine gave the best results for
chloroquine resistant malaria
(102)
. Moreover, berberine
sulphate was comparable to metronidazole as regards
potency on the growth of
T. vaginalis in vitro
(103)
.
Azadirachta indica, Melia Azadirachta; Neem
The major active constituents in neem are fatty
acids and terpenoids such as azadirachtin, which
is considered to possess anti-microbial and insect
repellent effects, among many other actions
(104)
.
Neem seed oil is problematic and should be kept
out of reach of children
(105)
. In this form, it is mainly
used in control of vector borne parasitic diseases.
The larvicidal and emergence inhibitory activities
of neem were tested successfully against the vectors
of malaria, lariasis and dengue fever
(106)
. Besides,
water-free neem seed extract shampoo was effective
against S. scabiei-infesting dogs in Egypt
(107)
. As an
acaricidal agent, neem extracts showed high level of
efcacy (80%) for adult ticks after 5 hours of
treatment
(108)
. As a pediculicide, its extract shampoo
proved to be highly effective against all stages of head
lice, with no side effects
(109)
. The signicant repellent
activities of neem against Phlebotomus papatasi
(110)
and
Ixodes ricinus
(111)
nymphs were reported.
Cinchona ofcinalis; Cinchona (Al-Quina tree)
The medicinally active constituents of cinchona are
a variety of alkaloids as quinine and quinidine.
Oral
quinine has marked side effects, including tinnitus,
dizziness and nausea
(112)
. Quinine has been used as
malaria treatment for more than 350 years in Africa
and remains the rst-line anti-malarial drug for the
treatment of complicated malaria in Europe and Africa.
Children with uncomplicated malaria are generally
treated with oral medication, except those unable to take
oral drugs
(113)
. It has substantial disadvantages mainly
its poor tolerability, the long treatment course and the
unpleasant
bitter taste of its tablets
(114)
. Therefore, it
is no longer recommended by the WHO as rst line
treatment for malaria and should be used only when
artemesinins are not available
(115)
. Treatment of S.
mansoni-infected female mice with daily intraperitoneal
injections of quinine and quinidine caused signicant
decrease in worm burden and egg production. Their
schistosomicidal effects
are due to their capacity
to interfere with hemozoin formation which is the
main heme detoxication pathway in S. mansoni
(116)
.
In addition, quinine and chloroquine are considered
potential drugs against L. loa infection
(117)
.
Triticum vulgare, Triticum aestivum; Wheat germ
(Ganin Habit Al-Kamh)
Lectin (wheat germ agglutinin) is the active constituent,
however, it is a concentrated source of vitamin E, folic
acid, phosphorus, thiamin, zinc and magnesium, as
well as essential fatty acids and fatty alcohols
(71)
. In a
clinical trial, wheat germ helped in quick resolution of
symptoms in Giardia-infected patients. It did not kill
the parasites, but prevented their growth, replication
and attachment
(118)
. In addition, the effectiveness of
wheat germ as a useful dietary supplement administered
to E. histolytica-infected patients was reported, both
in acute and chronic stages of infection
(119)
. When its
therapeutic effect was experimentally evaluated in
immunosuppressed mice infected with Cryptosporidium
oocysts, it
showed signicant reduction of excreted
oocysts on day 7 post-infection
(120)
. It was also shown to
have remarkable inhibitory effect on multiplication and
motility of T. Vaginalis
(87)
.
Cinnamomum zeylanicum; Cinnamon (Al-Kerfa)
Various terpenoids (eugenol and cinnamaldehyde)
are believed to account for cinnamon's medicinal
effects
(121)
. Some people develop bronchial constriction
or skin rash after exposure to cinnamon and chronic
use of the concentrated oil may cause mouth
inammation. Cinnamon is not recommended for
use by pregnant women
(71)
. Cinnamon essential oil
had potent insecticidal and ovicidal activities against
human head louse, P. humanus capitis
(122)
, acaricidal
activity against adult Dermanyssus gallinae
(123)
and
strong larvicidal activity against 4
th
instar Aedes
aegypti larvae
(124)
. Recently, its inhibitory action against
other mosquito species as Aedes albopictus, Culex
quinquefasciatus and Armigeres subalbatus larvae was
reported
(125)
.
 
 
 
Herbal Medicine and Parasites
8
Melaleuca alternifolia; Tea Tree:
Oil of tea tree containing terpenoids such as terpinen-4-
ol and cineole has the ability to kill resistant fungus and
bacteria
(126)
. The reported side effects included burning
and allergic reactions
(127)
. Its oil was reported to have
similar repellent effects, as clove oil used against scrub
typhus
(94)
. Tea tree oil (5%) showed
similar effects as
those of benzyl benzoate, lindane and ivermectin against
all stages of S. scabiei
(128)
. Acaricidal and pediculocidal
activity against house dust mites and lice was evaluated
using three essential oils: Tea tree, lavender and lemon
and the results revealed that tea tree oil was the most
effective
(129)
. Moreover, improvement of symptoms and
dramatic resolution of ocular irritation
and conjunctival
inammation were reported in patients with Demodex
blepharitis who were treated with 50% tea tree oil
shampoo for a minimum of 6 weeks
(130)
.
Cucurbita pepo, Cucurbita maxima; Pumpkin
(Karh El-Asal, or Al-Yakten):
Pumpkin seeds contain several active constituents:
Essential fatty acids, amino
acids, phytosterols (e.g.
β-sitosterol), minerals, vitamins and curcurbitin which
shows anti-parasitic activity
(131)
. Oral administration
of crude ethanolic extracts from C. maxima reduced
by 50% the levels of parasitaemia in Plasmodium
berghei-infected mice
(132)
. In heterophyiasis, a combined
extract of areca nut and pumpkin seeds gave an excellent
result than when either extract was given alone
(133)
.
In addition, it gave effective results against canine
tapeworms
(134)
. Its leaf extract with different solvents
showed larvicidal, ovicidal and repellent activities
against Culex quinquefasciatus
(135)
.
Less Commonly Used Herbal Medicine with
Antiparasitic Effects
Cuminum cyminum; Cumin (Al-Kammoun):
Cuminaldehyde, cymene and terpenoids are the major
constituents of volatile oils of cumin
(136)
. It is usually
used in traditional medicine as a stimulant, a carminative
and an astringent
(137)
. Signicant larvicidal activity
(100% mortality) of essential oils derived from cumin
against early 4
th
stage larvae of Aedes aegypti and Culex
pipiens was reported
(138)
.
Eugenia caryophyllata; Syzygium aromaticum
Clove (Al-Kornfel): The main constituents of the
clove essential oil are phenylpropanoids such as
carvacrol, thymol, eugenol and cinnamaldehyde
(139)
.
Under eld conditions, Gel B (20% clove oil) provided
signicant repellent effects against Aedes aegypti,
Culex quinquefasciatus and Mansonia uniformis. These
promising results raised the possibility for its use by
low-income rural communities against various mosquito
species
(140)
. Moreover, essential oils of clove showed
repellent effects against host-seeking chiggers (scrub
typhus) of Leptotrombidium imphalum
(94)
.
Pimpinella anisum; Anise (Al-Yanson): The active
constituents in anise, particularly the terpenoid anethole,
are in its volatile oil
(141)
. Its uses for medication in
parasitic diseases are due to its insecticidal and repellent
effects. The acaricidal activity of anise seed oil against
house dust mites Dermatophagoides farinae and
D. pteronyssinus was about 8.4 and 6.7 times more
toxic than benzyl benzoate, respectively
(142)
. Besides
its acaricidal activity, it was highly effective as both
larvicidal and ovicidal against different mosquito spp.
As Anopheles, Aedes and Culex
(95)
. Moreover, its spray
preparation had
signicant cure rates against head louse
infestations
(143)
.
Punica granatum; Pomegranate (Al-Roman): Ellagic
acid is likely to be the main constituent responsible for
the antiparasitic effect of pomegranate
(144)
. It acts through
stimulation of the cell-mediated and humoral components
of the immune system
(145)
. The in vitro studies conducted
in 2009 proved that ellagic acid may contribute as a
promising anti-malarial drug
(146)
, it had the ability to
inhibit the growth of P. falciparum asexual blood stages in
vitro
(147)
. In addition, a natural plant extract puried from
pomegranate was effective against T. vaginalis in vitro
(67)
.
Ricinus communis; Castor Oil Plant (El-Khirwa):
Ricinoleic acid is the main component of castor oil,
and it constitutes approximately 90% of its fatty acid
content
(148)
. Leaves of castor oil plant are toxic to sand
ies and offer protection against their bites and decrease
the risk of leishmaniasis
(149)
. The methanol extract of castor
oil exhibited acaricidal and insecticidal activities against
Haemaphysalis bispinosa and Hippobosca maculata
(158)
.
Rosmarinus ofcinalis; Rosemary (Ekleel Al-Gabal):
The active constituents include volatile oil (eucalyptol
or cineole) as a potent antibacterial agent, rosmarinic
acid with antioxidant activity and carnosol as anti-cancer
formation in animal studies. Internal intake of its oil
should be avoided during pregnancy because it may cause
abortion
(151)
. Rosemary oil produced decreased oviposition,
had high larvicidal activity against all larval stages of A.
aegypti and proved to be a promising agent in mosquito
control programs
(152)
Salix alba; Willow (Al-Sefsaf): The main active constituent
is glycoside salicin which has anti-inammatory and pain-
relieving actions. As with aspirin, some people may
experience stomach upset from taking willow
(153)
. During
the American Civil War, the unreliable supply and high cost
of quinine forced the Confederate Army to use alternative
treatments for malaria which were made from indigenous
plants such as dogwood, willow and tulip tree. The quinine
substitutes were generally considered useful but not as
effective as quinine
(154)
.
Thymus vulgaris; Thyme (Al-Zaatar): The primary
constituents are the volatile oils, including phenols, thymol
and carvacol. Thyme oil should be reserved for topical
 
 
Etewa and Abaza
9
use, as internally it may lead to dizziness, vomiting and
breathing difculties
(151)
. So, its hydroalcoholic extract
was signicantly effective in reduction of ulcer size in
cutaneous leishmaniasis
(155)
. Its volatile oil was effective
against Lucilia sericata 3
rd
stage larvae
(156)
and Pediculus
humanus
(157)
.
Herbal Medicine with Limited Molluscicidal Effects
Ambrosia maritima (Damsissa): In a eld trial in Egypt,
the possible use of single annual application of damsissa
plant, in snail control program for schistosomiasis
transmission season in Lower Egypt, was suggested
(158)
.
Longer prepatent period and remarkable decrease in
cercarial production was also recorded in snails treated
with the sublethal concentrations of this molluscicide
(159)
.
In addition, it has lethal effects on Lymnaea snails
(160)
.
Azolla pinnata: Molluscicidal activity of A. pinnata
was reported against B. alexandrina snails. Its ethanol
extract, at 6600 ppm for three hours
exposure, killed
100% and 19.4% of S. mansoni miracidia and cercariae,
respectively(161).
Euphorbia spp. (Sabbaar): The physiological and lethal
effects of the latex components of E. splendens(162)and
E. milii(163), on Biomphalaria spp. were reported. It was
found that unltered crude latex extract is a more potent
molluscicide than ltered latex(162) and milin (the active
component) is likely to be responsible for alteration of
normal physiological functions and lethality of snails(163).
Acknowledgment: The authors would like to thank Prof.
Dr. Raefa Darwish and Prof. Dr. Soad Nada, Parasitology
Department, Faculty of Medicine, Zagazig University,
for revising the review article and Eman Magdy for
references collection.
REFERNCES
1. WHO. http://www.who.int/mediacentre/factsheets/fs134/
en/; December, 2008.
2. Aggarwal BB, Sundaram C, Malani N, Ichikawa
H. Curcumin: The Indian solid gold. Adv Exp Med
Biol; 2007, 595: 1-75.
3. Abdulelah HAA, Zainal-Abidin BAH. In vivo anti-malarial
tests of Nigella sativa (Black seed) different extracts. Am J
Pharmacol Toxicol; 2007, 2: 46-50.
4. Acharya D, Shrivastava A. Indigenous Herbal Medicines:
Tribal formulations and traditional herbal practices.
Aavishkar Publishers Distributor, Jaipur, India; 2008, 440:
252-7.
5. WHO. Prevention and control of schistosomiasis and
soil-transmitted helminthiasis. Report of a WHO Expert
Committee; 2002, WHO Tech Rep Ser No. 912.
6. Greenwood BMK, Bojang CJ, Whitty M, Targett GA.
Malaria. Lancet; 2005, 365: 1487-98.
7. Croft SL, Sundar S, Fairlamb AH. Drug resistance in
leishmaniasis. Clin Microbiol Rev; 2006, 19: 111–26.
8. Anthony JP, Fyfe L, Smith H. Plant active components:
A resource for antiparasitic agents? Trends Parasitol;
2005, 21 (10): 462-8.
9. Elvin LM. Should we be concerned about herbal remedies?
J Ethnopharmacol; 2001, 75: 141-164.
10. Feldberg RS, Chang SC, Kottik AN. In vitro mechanism
of inhibition of bacterial growth by Allicin. Antimicrob
Agents Chemother; 1988, 32: 1763-1768.
11. Saleheen D, Ali SA, Yasinzai MM. Antileishmanial
activity of aqueous onion extract in vitro.
Fitoterapia; 2004, 75(1):9-13
12. Ahmed SA. In vitro effects of aqueous garlic
(Allium sativum) and onion (Allium cepa) extracts on
trichomonas vaginalis. PUJ; 2010, 3 (1&2): 45-54.
13. Abu El-Ezz NM. Effect of Nigella sativa and Allium
cepa oils on Trichinella spiralis in experimentally
infected rats. J Egypt Soc Parasitol; 2005, 35 (2): 511-23.
14. Stozkowska W, Raczyńska K. Efciency and application
safety of Cepan cream: Observation of a new indication.
Przegl Lek; 2008, 65 (5): 241-3.
15. Mantawy MM, Mahmoud AH. Effect of Allium cepa and
Allium sativum feeding on glucose, glycogen, protein
bands prole and phenol oxidase activity in Biomphalaria
alexandrina. J Egypt Soc Parasitol; 2002, 32 (1): 271-83.
16. Valdivieso R, Subiza J, Varela-Losada S. Bronchial
asthma, rhinoconjunctivitis and contact dermatitis caused
by onion. J Allergy Clin Immunol; 1994, 94: 928-30.
17. Ayaz E, Alpsoy HC. Garlic (Allium sativum) and traditional
medicine. Turkiye Parazitol Derg; 2007, 31 (2): 145-9.
18. Ankri S, Mirelman D. An antimalarial extract from neem
leaves is antiretroviral: Antimicrobial properties of allicin
from garlic. Microbe Infect; 1999, 1: 125-9.
19. Davis SR. An overview of the antifungal properties
of allicin and its breakdown products: The possibility
of a safe and effective antifungal prophylactic.
Mycoses; 2005, 48 (2): 95-100.
20. Soffar SA, Mokhtar GM. Evaluation of the antiparasitic
effect of aqueous garlic (Allium sativum) extract in
Hymenolepiasis nana and giardiasis. J Egypt Soc
Parasitol; 1991, 21: 497-502.
21. Harris JC, Plummer S, Turner MP, Lloyd D.
The microaerophilic agellate Giardia intestinalis:
Allium sativum (garlic) is an effective antigiardial.
Microbiology; 2000, 146 (12): 3119-27.
22. Jimenez JC, Uzcanga G, Zambrano A. Identication and
partial characterization of excretory/secretory products
with proteolytic activity in Giardia intestinalis. J Parasitol;
2000, 86: 859-62.
23. El-Nahas N, El-Melegy M, Abdel-Wahed M. Effect of
garlic on experimental cyclosporiasis. Egypt J Med Sci;
2003, 24 (1): 53-64.
 
 
Herbal Medicine and Parasites
10
24. Coppi A, Cabinian M, Mirelman D, Sinnis P.
Antimalarial activity of allicin, a biologically active
compound from garlic cloves. Antimicrob Agents
Chemother; 2006, 50 (5): 1731-7.
25. Ghazanfari T, Hassan ZM, Khamesipour A. Enhancement
of peritoneal macrophage phagocytic activity against
Leishmania major by garlic (Allium sativum) treatment. J
Ethnopharmacol; 2006, 103 (3): 333-7.
26. Gamboa LMR, Aranda GI, Mut MM, García MMR,
Dumonteil E. In vivo and in vitro control of Leishmania
mexicana due to garlic-induced NO production. Scand J
Immunol; 2007, 66 (5): 508-14.
27. Toulah FH, Al-Rawi MM. Efcacy of garlic extract
on hepatic coccidiosis in infected rabbits (Oryctolagus
cuniculus): Histological and biochemical studies. J Egypt
Soc Parasitol; 2007, 37 (3): 957-68.
28. Polat ZA, Vural A, Ozan F, Tepe B, Ozcelik S, Cetin A.
In vitro evaluation of the amoebicidal activity of garlic
(Allium sativum) extract on Acanthamoeba castellanii and
its cytotoxic potential on corneal cells. J Ocul Pharmacol
Ther; 2008, 24 (1): 8-14.
29. El-Shenawy NS, Soliman MF, Reyad SI. The effect
of antioxidant properties of aqueous garlic extract and
Nigella sativa as anti-schistosomiasis agents in mice.
Rev Inst Med Trop Sao Paulo; 2008, 50 (1): 29-36.
30. Riad NHA, Taha HAT, Mahmoud YI. Effects of garlic on
albino mice experimentally infected with Schistosoma
mansoni: A parasitological and ultrastructural study.
Trop Biomed; 2009, 26 (1): 40-50.
31. Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR. In vitro
screening of different Allium sativum extracts on hydatid
cysts protoscoleces. J Invest Surg; 2008, 21 (6): 318-22.
32. Valerio L, Maroli M. Evaluation of repellent and anti-
feeding effect of garlic oil (Allium sativum) against the
bite of Phlebotomine sandies (Diptera: Psychodidae).
Ann Ist Super Sanita; 2005, 41 (2): 253-6.
33. Birrenkott GP, Brockenfelt GE, Greer JA, Owens MD.
Topical application of garlic reduces northern fowl mite
infestation in laying hens. Poult Sci; 2000, 79(11): 1575-7.
34. Singh A, Singh DK. Effect of herbal molluscicides
and their combinations on the reproduction of the
snail Lymnaea acuminata. Arch Environ Contam
Toxicol; 2004, 46 (4): 470-7.
35. AlKadi HO. Antimalarial drug toxicity: A review.
Chemotherapy; 2007, 53:385-91.
36. Leung AY, Foster S. Encyclopedia of Common Natural
Ingredients Used in Food, Drugs, and Cosmetics. 2nd
Edition, John Wiley and Sons, New York; 1996, 3: 382.
37. McGuffun M, Hobbs C, Upton R, Goldberg A. American
Herbal Products Association's Botanical Safety Handbook.
CRC Press, Boca Raton, FL; 1997, 15.
38. Song J, Socheat D, Tan B et al. Rapid and effective
malaria control in Cambodia through mass administration
of artemisinin-piperaquine. Malar J; 2010, 23: 9-57.
39. Dondorp AM, Yeung S, White L et al. Artemisinin
resistance: Current status and scenarios for containment.
Nat Rev Microbiol; 2010, 8 (4): 272-80.
40. Posner GH, O'Neill PM. Knowledge of the proposed
chemical mechanism of action and cytochrome p450
metabolism of antimalarial trioxanes like artemisinin
allows rational design of new antimalarial peroxides.
Acc Chem Res; 2004, 37 (6): 397-404.
41. Woodrow CJ, Planche T, Krishna S. Artesunate versus
quinine for severe falciparum malaria. Lancet; 2006, 14
(9505): 110-1.
42. Golenser J, Waknine JH, Krugliak M, Hunt NH, Grau
GE. Current perspectives on the mechanism of action of
artemisinins. Int J Parasitol; 2006, 36: 1427–41.
43. Sen R, Bandyopadhyay S, Dutta A et al. Artemisinin
triggers induction of cell-cycle arrest and apoptosis
in Leishmania donovani promastigotes. J Med
Microbiol; 2007, 56 (9): 1213-8.
44. Nagamune K, Moreno SN, Sibley LD. Artemisinin-
resistant mutants of Toxoplasma gondii have
altered calcium homeostasis. Antimicrob Agents
Chemother; 2007, 51 (11): 3816-23.
45. Dunay IR, Chan WC, Haynes RK, Sibley LD.
Artemisone and artemiside control acute and reactivated
toxoplasmosis in a murine model. Antimicrob Agents
Chemother; 2009, 53 (10): 4450-6.
46. D'Angelo JG, Bordón C, Posner GH, Yolken R, Jones-
Brando L. Artemisinin derivatives inhibit Toxoplasma
gondii in vitro at multiple steps in the lytic cycle. J
Antimicrob Chemother; 2009, 63 (1): 146-50.
47. Yang YQ, Xiao SH, Tanner M et al. Histopathological
changes in juvenile Schistosoma haematobium
harboured in hamsters treated with artemether.Acta
Trop; 2001, 79: 135-41.
48. Xiao SH, Shen BG, Tzinger JU, Chollet J, Tanner
M. Ultrastructural alterations in adult Schistosoma
mansoni caused by artemether. Mem Inst Oswaldo
Cruz; 2002, 97: 717–24.
49. El-Bassiouni EA, Helmy MH, Saad EI, Abdel-El-Nabi
KM, Abdel-Meguid E, Hussein HS. Modulation of the
antioxidant defence in different developmental stages of
Schistosoma mansoni by praziquantel and artemether. Br J
Biomed Sci; 2007, 64 (4): 168-74.
50. Keiser J, Rinaldi L, Veneziano V et al. Efcacy and safety
of artemether against a natural Fasciola hepatica infection
in sheep. Parasitol Res; 2008, 103 (3): 517-22.
51. O'Neill JF, Johnston RC, Halferty L, Brennan GP, Keiser
J, Fairweather I. Adult triclabendazole-resistant Fasciola
hepatica: Morphological changes in the tegument and
gut following in vivo treatment with artemether in the rat
model. J Helminthol; 2009, 83 (2): 151-63.
 
 
Etewa and Abaza
11
52. Caner A, Doskaya M, Degirmenci A, Can H, Baykan
S. Comparison of the effects of Artemisia vulgaris and
Artemisia absinthium growing in western Anatolia
against trichinellosis (Trichinella spiralis) in rats.
Exp Parasitol; 2008, 119 (1): 173-9.
53. Jaenson TG, Palsson K, Borg-Karlson AK. Evaluation
of extracts and oils of tick-repellent plants from Sweden.
MedVet Entomol; 2005, 9 (4): 345-52.
54. Mills SY. Out of the Earth: The Essential Book of Herbal
Medicine. Middlesex, UK: Viking Arkana, 1991: 500-2.
55. Badria F, Abou-Mohamad G, El-Mowafy A, Masoud
A, Salama T. Mirazid: A new schistosomicidal drug.
Pharmaceut Biol; 2001, 39: 127-131.
56. Massoud AM, El-Ebiary FH, Abou-Gamra MM, Mohamed
GF, Shaker SM. Evaluation of schistosomicidal activity
of myrrh extract: Parasitological and histological study. J
Egypt Soc Parasitol; 2004, 34 (3): 1051-76.
57. Hamed MA, Hetta MH. Efcacy of Citrus reticulata
and Mirazid in treatment of Schistosoma mansoni.
Mem Inst Oswaldo Cruz; 2005, 100: 771–8.
58. Abo-Madyan AA, Morsy TA, Motawea SM Efcacy
of Myrrh in the treatment of schistosomiasis
(haematobium and mansoni) in Ezbet El-Bakly, Tamyia
Center, El-Fayoum Governorate. Egypt. J Egypt Soc
Parasitol, 34: 423-46.
59. Botros S, William S, Ebeid FD, Cioli N, Day TA.
Lack of evidence for an antischistosomal activity
of myrrh in experimental animals, Am J Trop Med
Hyg; 2004, 71: 206–10.
60. Barakat R, El-Morshedy H, Fenwick A. Efcacy of myrrh
in the treatment of human schistosomiasis mansoni.
Am J Trop Med Hyg; 2005, 73: 365-7.
61. Mahmoud MS, Dobal SA, Soliman K. Immune response
in Fasciola gigantica experimentally infected rabbits
treated with either carnosine or Mirazid®. Res J Parasitol;
2008, 3: 40-9.
62. Botros SS, El-Lakkany NM, Badawy AA, Mahmoud
SS, Ebeid FA, Fenwick A. Mirazid shows insignicant
activity against ovine fascioliasis. Ann Trop Med
Parasitol; 2009, 103 (7): 605-16.
63. Al-Mathal EM, Fouad MA. Myrrh (Commiphora
molmol) in treatment of human and sheep
dicrocoeliasis dendriticum in Saudi Arabia. J Egypt Soc
Parasitol; 2004, 34 (2): 713-20.
64. Massoud AM, El-Shazly AM, Morsy TA. Mirazid
(Commiphora molmol) in treatment of human
heterophyiasis. J Egypt Soc Parasitol, 37 (2): 395-410.
65. Massoud, AM, Shazly AM, Shahat SA, Morsy TA.
Mirazid in treatment of human hymenolepiasis. J Egypt
Soc Parasitol; 2007, 37 (3): 863-76.
66. Massoud AM, Hafez AO, Abdel-Gawad AG, El-Shazly
AM, Morsy TA. Mirazid alone or combined with
Paromomycin in treating cryptosporidiosis parvum in
immunocom-petent hospitalized patients. J Egypt Soc
Parasitol; 38 (2): 399-418.
67. El-Sherbini GT, El-Gozamy BR, Abdel-Hady NM,
Morsy TA. Efcacy of two plant extracts against vaginal
trichomoniasis. J Egypt Soc Parasitol; 2009, 39(1): 47-58.
68. Massoud AM, Labib IM. Larvicidal activity of
Commiphora molmol against Culex pipiens and Aedes
caspius larvae. J Egypt Soc Parasitol; 2000, 30(1): 101-15.
69. Allam AF, El-Sayad MH, Khalil SS. Laboratory
assessment of the molluscicidal activity of Commiphora
molmol (Myrrh) on Biomphalaria alexandrina,
Bulinus truncatus and Lymnaea cailliaudi. J Egypt
Soc Parasitol; 31: 683-90.
70. Massoud AM, Metwally DM, Khalifa KE, Habib
FS. Compatibility of Biomphalaria alexandrina
snails to infection with Schistosoma mansoni after
exposure to sublethal concentrations of Myrrh. J Egypt
Soc Parasitol; 34: 995–1008.
71. Blumenthal M, Busse WR, Goldberg A. The Complete
Commission E Monographs: Therapeutic guide to
herbal medicines. Boston, MA, Integrative Medicine
Communications; 1998; 309.
72. Charles V, Charles SX. The use and efcacy of
Azadirachta indica ADR ('Neem') and Curcuma longa
('Turmeric') in scabies: A pilot study. Trop Geogr
Med; 1992, 44(1-2):178-81
73. Waknine-Grinberg JH, McQuillan JA, Hunt N, Ginsburg
H, Golenser J. Modulation of cerebral malaria by
fasudil and other immune-modifying compounds.
Exp Parasitol; 2010, 125 (2): 141-6
74. Nose M, Koide T, Ogihara Y, Yabu Y, Ohta N.
Trypanocidal effects of curcumin in vitro. Biol Pharm
Bull; 1998, 21 (6): 643-5.
75. Koide T, Nose M, Ogihara Y. Leishmanicidal Effect of
Curcumin in Vitro. Biol Pharm Bull; 2002, 25 (1): 131-3.
76. Pérez-Arriaga L, Mendoza-Magaña ML, Cortés-Zárate
R et al. Cytotoxic effect of curcumin on Giardia lamblia
trophozoites. Acta Trop; 2006, 98 (2): 152-161.
77. Al-Zanbagi NA, Zelai NT. Two methods for attenuating
Toxoplasma gondii tachyzoites RH strain by using
ethanol extract of Curcuma longa. J Egypt Soc
Parasitol; 2008, 38 (3): 965-76.
78. El-Ansary AK, Ahmed SA, Aly SA. Antischistosomal
and liver protective effects of Curcuma longa extract
in Schistosoma mansoni infected mice. Ind J Exp
Biol; 2007, 45 (9): 791-801.
79. Allam G. Immunomodulatory effects of curcumin
treatment on murine schistosomiasis mansoni.
Immunobiology; 2009, 214 (8): 712-27.
80. Boonjaraspinyo S, Boonmars T, Aromdee C et al. Turmeric
reduces inammatory cells in hamster opisthorchiasis.
Parasitol Res; 2009, 105 (5): 1459-63.
81. Omran NE, El-Nouby KA, Shoheib ZS, Kabbash
AM. Molluscicidal effects of some plant extracts
on Biomophlaria alexandrina, the intermediate host
snail of Schistosoma mansoni in Egypt. J Egypt
Ger Zool; 2007, 53: 1-29.
 
 
Herbal Medicine and Parasites
12
82. Boskabady MH. Effect of Nigella Sativa on isolated
guinea pig trachea. Arch Iran Med; 2002, 5 (2): 103-7.
83. EL-Dakhakhny M, Mady NI, Halim MA. Nigella
sativa oil protects against induced hepatotoxicity
a n d i m p r o v e s s e r u m l i p i d p r o  l e i n r a t s . A r z n e i m i t
Forsch; 2000, 50: 832-6.
84. Mady NI, Abdel-Aziem T, Matta M. Effect of combination
of Nigella sativa oil and glibenclamide on some metabolic
parameters and oxidative changes in streptozocin-induced
diabetic rats (in vivo and in vitro study). J Egypt Pharmacol
Exp Ther; 2001, 20: 359-83.
85. Ayaz E, Yilmaz H, Ozbek H, Tas Z, Orunc O. The effect
of Nigella sativa oil against Aspiculuris tetraptera
and Hymenolepis nana in naturally infected mice.
Saudi Med J; 2007, 28 (11): 1654-7.
86. El-Wakil HS. Evaluation of the in vitro effect of Nigella
sativa aqueous extract on Blastocystis hominis isolates. J
Egypt Soc Parasitol; 2007, 37 (3): 801-13.
87. Ahmed MA. In vitro inhibition of Trichomonas vaginalis
growth by WGA and Nigella sativa. New Egypt J
Med; 2008, 39 (1): 156-68.
88. Ghayur MN, Gilani AH. Pharmacological basis for the
medicinal use of ginger in gastrointestinal disorders.
Dig Dis Sci; 2005, 50: 1889-97.
89. Sohni YR, Kaimal P, Bhatt RM. The antiamoebic
effect of a crude drug formulation of herbal extracts
against Entamoeba histolytica in vitro and in vivo. J.
Ethnopharmacol; 1995, 45 (1): 43-52.
90. Sanderson L, Bartlett A, Whiteld PJ. In vitro and in
vivo studies on the bioactivity of a ginger (Zingiber
ofcinale) extract towards adult schistosomes and their
egg production. J Helminthol; 2002, 76(3):241-7.
91. Iqbal Z, Lateef M, Akhtar MS, Ghayur MN,
Gilani AH. In vivo anthelmintic activity of ginger
against gastrointestinal nematodes of sheep.
J Ethnopharmacol; 2006, 106 (2): 285-7.
92. El-Melegy MA, El-Saify GH, Hassab-El-Nabi SE.
Evaluation of the therapeutic effect of ginger compared
to ubendazole on experimental trichinellosis in mice.
Egypt J Med Sci; 2006, 27 (2): 25-48.
93. Merawin LT, Arifah AK, Sani RA et al. Screening of
microlaricidal effects of plant extracts against Dirolaria
immitis. Res Vet Sci.; 2010, 88 (1): 142-7.
94. Eamsobhana P, Yoolek A, Kongkaew W et al. Laboratory
evaluation of aromatic essential oils from thirteen plant
species as candidate repellents against Leptotrombidium
chiggers (Acari: Trombiculidae), the vector of scrub
typhus. Exp Appl Acarol; 2009, 47 (3): 257-62.
95. Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SP.
Insecticidal, repellent and oviposition-deterrent activity
of selected essential oils against Anopheles stephensi,
Aedes aegypti and Culex quinquefasciatus. Bioresour
Technol; 2005, 96 (16): 1749-57.
96. Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha
K, Bagavan A. Mosquito larvicidal activity of isolated
compounds from the rhizome of Zingiber ofcinale.
Phytother Res; 22 (8): 1035-9.
97. Adewunmi CO, Oguntimein BO, Furu P. Molluscicidal
and antischistosomal activities of Zingiber ofcinale.
Planta Med; 1990, 56 (4): 374-6.
98. Singh VK, Singh S, Singh DK. Effect of active
molluscicidal component of spices on different
enzyme activities and biogenic amine levels in the
nervous tissue of Lymnaea acuminata. Phytother
Res; 1999, 13 (8): 649-54.
99. Ghosh AK, Bhattacharyya FK, Ghosh DK. Leishmania
donovani: Amastigote inhibition and mode of action of
berberine. Exp Parasitol; 1985, 60 (3): 404-13.
100. Ahuja A, Purohit SK, Yadav JS, Netra PR. Cutaneous
leishmaniasis in domestic dogs. Ind J Public
Health; 1993, 37 (1): 29-31.
101. Sohni YR, Bhatt RM. Activity of a crude
extract formulation in experimental hepatic
amoebiasis and in immunomodulation studies. J
Ethnopharmacol; 1996, 54 (2-3): 119-24.
102. Sheng WD, Jiddawi MS, Hong XQ, Abdulla SM.
Treatment of chloroquine-resistant malaria using
pyrimethamine in combination with berberine, tetracycline
or cotrimoxazole. East Afr Med J; 1997, 74 (5): 283-4.
103. Soffar SA, Metwali DM, Abdel-Aziz SS, El-Wakil
HS, Saad GA. Evaluation of the effect of a plant
alkaloid (berberine derived from Berberis aristata)
on Trichomonas vaginalis in vitro. J Egypt Soc
Parasitol; 2001, 31 (3): 893-904.
104. Rembold H. The azadirachtins-their potential for
insect control. Econ Med Plant Res; 1989, 3:57-72.
105. Sinniah D, Baskara G, Looi LM, Leong KL. Reye-
like syndrome due to margosa oil poisoning:
R e p o r t o f a c a s e w i t h p o s t m o r t e m  n d i n g s .
Am J Gastroenterol; 1982, 77: 158-161.
106. Gunasekaran K, Vijayakumar T, Kalyanasundaram M.
Larvicidal and emergence inhibitory activities of Neem
Azal T/S 1.2% EC against vectors of malaria, lariasis
and dengue. Indian J Med Res; 2009, 130 (2): 138-145.
107. Abdel-Ghaffar F, Al-Quraishy S, Sobhy H, Semmler
M. Neem seed extract shampoo (Wash Away
Louse): An effective plant agent against Sarcoptes
scabiei mites infesting dogs in Egypt. Parasitol
Res; 2008, 104 (1): 145-8.
108. Srivastava R, Ghosh S, Mandal DB et al. Efcacy
of Azadirachta indica extracts against Boophilus
microplus. Parasitol Res; 2008, 104 (1): 149-153.
109. Abdel-Ghaffar F, Semmler M. Efcacy of neem seed
extract shampoo on head lice of naturally infected
humans in Egypt. Parasitol Res; 2007, 100 (2): 329-32.
110. Srinivasan R, Kalyanasundaram M. Relative efcacy
of DEPA and neem oil for repellent activity against
 
 
Etewa and Abaza
13
Phlebotomus papatasi, the vector of leishmaniasis. J
Commun Dis; 2001, 33 (3): 180-4.
111. Garboui SS, Jaenson TG, Pålsson K. Repellency
of MyggA Natural spray (para-menthane-3, 8-diol)
and RB86 (neem oil) against the tick Ixodes ricinus
(Acari: Ixodidae) i n t h e  e l d i n e a s t - c e n t r a l S w e d e n .
Exp Appl Acarol; 2006, 40 (3-4): 271-7.
112. Taylor WR, White NJ. Antimalarial drug toxicity:
A review. Drug Saf; 2004, 27: 25–61.
113. Kayitare E, Vervaet C, Mehuys E et al. Taste-masked
quinine pamoate tablets for treatment of children
with uncomplicated Plasmodium falciparum malaria.
Int J Pharmacol; 2010, 392 (1-2): 29-34.
114. Kremsner PG, Winkler S, Brandts M, Neifer CS.
Clindamycin in combination with chloroquine or
quinine is an effective therapy for uncomplicated
Plasmodium falciparum malaria in children from Gabon.
J Infect Dis; 1994, 169:467-70.
115. Achan J, Tibenderana JK, Kyabayinze D. Effectiveness
of quinine versus artemether-lumefantrine for treating
uncomplicated falciparum malaria in Ugandan
children. Brit Med J; 2009, 338: 2763.
116. Corrêa SJB, Menezes D, Vannier-Santos MA, Ferreira-
Pereira A, Almeida GT. Interference with hemozoin
formation represents an important mechanism of
schistosomicidal action of antimalarial quinoline
methanols. PLos Negl Trop Dis; 2009, 3 (7): 477.
117. Kamgno J, Djomo PN, Pion SD, Thylefors B, Boussinesq
M. A controlled trial to assess the effect of quinine,
chloroquine, amodiaquine and artesunate on Loa loa
microlaremia. Am J Trop Med Hyg; 2010, 82(3): 379-85.
118. Grant J, Mahanty S, Khadir A. Wheat germ supplement
reduces cyst and trophozoite passage in people with
giardiasis. Am J Trop Med Hyg; 2001, 65: 705-10.
119. Eassa AHA, Ismail MAM, Younis AIH, El-Wakil SS.
Effect of wheat germ supplement in patients with
intestinal amoebiasis. Presented in the 22nd Conference
of the Egyptian Society Of Basic Medical Sciences, 17th,
April 2003, Cairo University, Cairo, Egypt.
120. Moustafa MA. Role of wheat germ agglutinin (WGA)
in treatment of experimental cryptosporidiosis. J Egypt
Soc Parasitol; 2003, 33 (2): 443-56.
121. Singh HB, Srivastava M, Singh AB, Srivastava
AK. Cinnamon bark oil, a potent fungitoxicant
against fungi causing respiratory tract mycoses.
Allergy; 1995, 50: 995-9.
122. Yang YC, Lee HS, Lee SH, Clark JM, Ahn YJ.
Ovicidal and adulticidal activities of Cinnamomum
zeylanicum bark essential oil compounds and related
compounds against Pediculus humanus capitis
(Anoplura: Pediculicidae). Int J Parasitol; 2005, 35 (14):
1595-600.
123. Kim SI, Yi JH, Tak JH, Ahn YJ. Acaricidal activity of
plant essential oils against Dermanyssus gallinae (Acari:
Dermanyssidae). Vet Parasitol; 2004, 120 (4): 297-304.
124. Cheng SS, Liu JY, Tsai KH, Chen WJ, Chang ST.
Chemical composition and mosquito larvicidal
activity of essential oils from leaves of different
Cinnamomum osmophloeum provenances. J Agricul Food
Chem; 2004, 52: 4395-400.
125. Cheng SS, Liu JY, Huang CG, Hsui YR, Chen WJ,
Chang ST. Insecticidal activities of leaf essential oils
from Cinnamomum osmophloeum against three mosquito
species. Bioresour Technol; 2009, 100 (1): 457-64.
126. Carson CF, Cookson BD, Farrelly HD, Riley T.
Susceptibility of methicillin-resistant Staphylococcus
aureus to the essential oil of Melaleuca alternifolia. J
Antimicrob Chemother; 1995, 35:421-4.
127. Knight TE, Hansen BM. Melaleuca oil (tea tree oil)
dermatitis. Med J Australia; 1994, 30: 423-27.
128. Walton SF, Myerscough MR, Currie BJ. Studies in
vitro on the relative efcacy of current acaricides for
Sarcoptes scabiei var. hominis. Trans R Soc Trop
Med Hyg; 2000, 94 (1): 92-6.
129. Canyon DV, Speare R. A comparison of botanical
and synthetic substances commonly used to prevent
head lice (Pediculus humanus var. capitis) infestation.
Int J Dermatol; 2007, 46 (4): 422-6.
130. Kheirkhah A, Casas V, Li W, Raju VK, Tseng SC.
Corneal manifestations of ocular Demodex infestation.
Am J Ophthalmol; 2007, 143 (5): 743-9.
131. Rybaltovskii OV. On the discovery of cucurbitin-a
component of pumpkin seed with anthelmintic action.
Med. Parazitol; 1966, 35: 487-8.
132. Amorim CZ, Marques AD, Cordeiro RS. Screening
of the antimalarial activity of plants of the
Cucurbitaceae family. Mem Inst Oswaldo Cruz; 1991, 86
(Suppl 2): 177-80.
133. Mahmoud LH, Basiouny SO, Dawoud HA. Treatment
of experimental heterophyiasis with two plant
extracts, areca nut and pumpkin seed. J Egypt Soc
Parasitol; 2002, 32 (2): 501-6.
134. Díaz OD, Lloja LL, Carbajal ZV. Preclinical studies
of Cucurbita maxima (pumpkin seeds): A traditional
intestinal antiparasitic in rural urban areas.
Rev Gastroenterol Peru; 2004, 24 (4): 323-327.
135. Mullai K, Jebanesan A. Larvicidal, ovicidal and repellent
activities of the leaf extract of two cucurbitacious plants
against larial vector Culex quinquefasciatus (Say)
(Diptera: Culicidae). Trop Biomed; 2007, 24 (1): 1-6.
136. El-Hamidi A, Ahmed SS. The content and composition of
some umbelliferous essential oils. Die.Pharmazie; 1966, 7:
438-9.
137. Allahghadri T, Rasooli I, Owlia P et al. Antimicrobial
property, antioxidant capacity and cytotoxicity
of essential oil from cumin produced in Iran.
J Food Sci; 2010, 75 (2): 54-61.
138. Lee HS. Mosquito larvicidal activity of aromatic medicinal
plant oils against Aedes aegypti and Culex pipiens pallens.
J Am Mosq Control Assoc; 2006, 22 (2): 292-5.
 
 
Herbal Medicine and Parasites
14
139. Chaieb K, Hajlaoui H, Zmantar T et al. The
chemical composition and biological activity of
clove essential oil, Eugenia caryophyllata (Syzigium
aromaticum L. Myrtaceae): A short review. Phytother
Res; 2007, 21 (6): 501-6.
140. Trongtokit Y, Rongsriyam Y, Komalamisra N, Krisadaphong
P, Apiwathnasorn C. Laboratory and eld trial of developing
medicinal local Thai plant products against four species
of mosquito vectors. Southeast Asian J Trop Med Public
Health; 2004, 35 (2): 325-33.
141. Weiss RF. Herbal Medicine. Beaconseld Publishers Ltd;
Gothenberg, Sweden, 1985, 203.
142. Lee HS. P-Anisaldehyde: Acaricidal component of
Pimpinella anisum seed oil against the house dust mites
Dermatophagoides farinae and Dermatophagoides
pteronyssinus. Planta Med; 2004, 70 (3): 279-81.
143. Burgess IF, Brunton ER, Burgess NA. Clinical trial
showing superiority of a coconut and anise spray over
permethrin 0.43% lotion for head louse infestation.
Eur J Pediatr; 2010, 169 (1): 55-62.
144. Soh PN, Witkowski B, Olagnier D, Nicolau MC. In vitro
and in vivo properties of ellagic acid in malaria treatment.
Antimicrob Agents Chemoth; 2009, 53: 1100–6.
145. Gracious R, Selvasubramanian RS, Jayasundar S.
Immunomodulatory activity of Punica granatum in
rabbits: A preliminary study. J Ethnopharmacol; 2001,
78:85-7.
146. Dell'Agli M, Galli GV, Corbett Y et al. Antiplasmodial
activity of Punica granatum L fruit rind. J Ethnopharmacol;
2009, 125 (2): 279-85.
147. Sturm N, Zimmermann Y, Hu HK, Wolf S.
Compounds structurally related to ellagic acid show
improved antiplasmodial activity. Antimicrob Agents
Chemoth; 2009, 53: 622-30.
148. Burdock GA, Carabin IG, Grifths